PHA.06116.01 - G-Both: Purification of Products from Biotechnological Processes (Downstream Processing) (Complete module description)

PHA.06116.01 - G-Both: Purification of Products from Biotechnological Processes (Downstream Processing) (Complete module description)

Original version English
PHA.06116.01 10 CP
Module label G-Both: Purification of Products from Biotechnological Processes (Downstream Processing)
Module code PHA.06116.01
Semester of first implementation
Faculty/Institute Institut für Pharmazie
Module used in courses of study / semesters
  • Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation valid from WS 2019/20 > Pflichtmodule
  • Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation (WS 2015/16 - SoSe 2023) > Pflichtmodule
Responsible person for this module
Further responsible persons
M. Pietzsch
Prerequisites
C-Both: Construction of production organisms;
D-Both: Introduction to Bioprocess Technology (Upstream Processing)
Skills to be acquired in this module
  • Knowledge of the basic concepts of downstream processing
  • Foundations of protein purification
  • Knowledge on equipment and design parameters
  • Knowledge of scale-up parameters
  • Challenges in prevention or elimination of contaminants
  • Knowledge on process integration: Strain development, up- and downstream processing
  • Basics of technical/industrial purification of proteins
  • Know how to plan and perform the purification of a target protein from biomass
Module contents
Course G.1: Introduction to Downstream Processing
  • Overview on potential contaminations of target products and strategies for their removal
  • Equipment: Design and operation
  • Cell disintegration
  • Solid-Fluid separations
  • Liquid-Liquid extraction
  • Precipitation and crystallization
  • Chromatographic separations
  • Special applications: Purification of membrane proteins, inclusion bodies, antibodies, viruses, DNA, etc.
Course G-Both.2: Project seminar on Downstream Processing
  • Purity control by SDS-PAGE and determination of specific enzymatic activity
  • Cell disintegration
  • Solid / Liquid Separation
  • Protein precipitation
  • Preparative chromatography for the purification of proteins
  • Packing of chromatography columns
  • Generation of purification tables
Forms of instruction Seminar (3 SWS)
Seminar (2 SWS)
Practical training (3 SWS)
Course
Languages of instruction German, English
Duration (semesters) 1 Semester Semester
Module frequency jedes Sommersemester
Module capacity unlimited
Time of examination
Credit points 10 CP
Share on module final degree Course 1: %; Course 2: %; Course 3: %; Course 4: %.
Share of module grade on the course of study's final grade 1
Module course label Course type Course title SWS Workload of compulsory attendance Workload of preparation / homework etc Workload of independent learning Workload (examination and preparation) Sum workload
Course 1 Seminar Project seminar 3 0
Course 2 Seminar Project seminar 2 0
Course 3 Practical training Lab course 3 0
Course 4 Course Private studies 0
Workload by module 300 300
Total module workload 300
Examination Exam prerequisites Type of examination
Course 1
Course 2
Course 3
Course 4
Final exam of module
Attestation on project seminar contents, Project seminar protocol, Oral presentation on a current research topic in downstream processing
Klausur
Exam repetition information
Prerequisites and conditions Prerequisites Frequency Compulsory attendance Share on module grade in percent
Course 1 Summer semester No %
Course 2 Summer semester No %
Course 3 Summer semester No %
Course 4 Summer semester No %