PHA.06110.02 - C-Both: Construction of production organisms - Hosts and vectors (Complete module description)
PHA.06110.02 - C-Both: Construction of production organisms - Hosts and vectors (Complete module description)
PHA.06110.02
10 CP
Module label
C-Both: Construction of production organisms - Hosts and vectors
Module code
PHA.06110.02
Semester of first implementation
Faculty/Institute
Institut für Pharmazie
Module used in courses of study / semesters
Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation valid from WS 2019/20 > Pflichtmodule
Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation (WS 2015/16 - SoSe 2023) > Pflichtmodule
Responsible person for this module
Further responsible persons
S. Schilling
Prerequisites
Skills to be acquired in this module
Knowledge on synthesis and structure/function relationship of DNA, RNA and protein
Basic principles of gene structure, regulatory elements, function and regulation of activity in biotechnologically important prokaryotic and eukaryotic organisms
Theoretical ability to cultivate bacteria, yeasts and phototrophic organisms
Knowledge about basic principles of gene cloning
Theoretical ability to construct a genetically modified organism by gene cloning und expression in a final production host
Insight into molecular techniques of plant and microalgae transformation
Survey of basic techniques of plant regeneration and concepts of in vitro plant cell, tissue and organ cultures
Overview of biotechnological application of phototrophic organisms and transgenic plants
Advantages of bacteria, yeast and insect cells as gene expression systems
Overview over products of molecular and classic biotechnology of microorganisms
Practical know-how for transfection and cultivation of microbial cells
Practical know-how on heterologous gene expression in microorganisms and plants
Practical knowhow in analyzing genetically modified plants
Module contents
Course C-Both.1: Methods and strategies for the generation of production strains
Overview biotechnologically relevant organisms: product related selection, advantages/disadvantages
Toolbox biotechnology: Gene analysis, DNA/protein sequencing, detection methods for products
Strategies for improvement of strains and products: random/site directed mutagenesis, protein tags
Examples for successful application in pharmaceutical and industrial biotechnology using different production organisms (bacteria, yeast, plants)
Course C-Both.2: Biotechnology of phototrophic organisms
Principal organization and expression of nuclear and organellar plant genomes
Plant and cell architecture, compartments and protein sorting
Strategies and procedures for plant regeneration and propagation
Plant cell, tissue and organ cultures: initiation and maintenance
Mechanism of Agrobacterium-mediated T-DNA transfer and applications
Plant transformation techniques and gene expression systems in plants
Vector design and optimization, stability and analysis of transgenes
Examples of molecular plant biotechnology: natural and novel products
Overview of (biotechnological relevant) micro- and macroalgae and application
The unicellular green algae Chlamydomonas reinhardtii as a model organism
Molecular biology of and gene technology with Cyanobacteria
Course C-Both.3: Molecular Biotechnology
Principal mechanisms of DNA synthesis in vitro and in vivo and use of enzymes for gene cloning
Mechanism of RNA and protein synthesis in bacteria
Structure and function of RNA and DNA
Regulation of gene expression in prokaryotes and eukaryotes (yeast) and use for heterologous expression of proteins
Methods of gene cloning and target gene isolation, library construction and screening
Theoretical ability to construct a genetically modified organism for heterologous protein production
Theoretical ability to cultivate bacteria and yeasts
Course C-Both.4: Project seminar on construction of hosts and vectors
Amplification of target DNA using PCR, restriction digest and vector ligation.
Transformation of E. coli with plasmid, selection, monitoring of the expression success.
Testing transgenic plants for the presence of transferred DNA
Monitoring heterologous protein production in plants
Share of module grade on the course of study's final grade
1
Module course label
Course type
Course title
SWS
Workload of compulsory attendance
Workload of preparation / homework etc
Workload of independent learning
Workload (examination and preparation)
Sum workload
Course 1
Lecture
Lecture
2
0
Course 2
Seminar
Project seminar
3
0
Course 3
Practical training
Lab course
4
0
Course 4
Course
Selbststudium
0
Workload by module
300
300
Total module workload
300
Examination
Exam prerequisites
Type of examination
Course 1
Course 2
Course 3
Course 4
Final exam of module
Examination on course contents part microorganisms, Protocol on course part microorganisms, Examination on course contents part plant cell technology, Protocol on course part plant cell technology