M. Pietzsch

Modules of the first semester (A-Both, B-PhBT, C-Both, D-Both)

Knowledge of drug delivery routes with focus on biotech products
Challenges of protein formulations
Knowledge of formulation processes and additives
Knowledge of drug delivery mechanisms and basic concepts of pharmacokinetics
Knowledge of parenteral controlled release formulations
Knowledge on posttranslational modifications of proteins in vivo
Rationale behind posttranslational modification of proteins
Know-how to synthetically modify proteins in vitro
Knowledge on the application of biocatalysts for the synthesis of drugs
Biosynthetic pathways of plant secondary metabolites
Regulatory mechanisms of metabolite production
Plant-derived pharmaceuticals
Strategies to produce metabolites in transgenic plants
Successful examples of pharmaceutical production in plants
Knowledge on the application of medical devices in `Regenerative Medicine`, Clarification of terms and definitions
Distinction of biomaterial classes and characteristic uses
Preparation of scaffolds, hydrogels and tailored surfaces
Understanding of benefits and limitations of tissue engineering

1. Course I-PhBT.1: Drug delivery

• Characteristics of application routes
• Basics of pharmacokinetics
• Principles of protein formulation techniques
• Rational based formulation development / Formulation Screening
• Stabilization principles for proteins
• Controlled Release: Principles, Materials and Kinetics

2. Course I-Ph.2: Covalent modifications of proteins

• Posttranslational modifications found in nature, e. g. glycosylation, farnesylation, phosphorylation, protein-protein cross-linking, protein splicing, oxidation, etc.
• Enzymes involved in posttranslational modification
• Bio- and chemo-catalysis for the in vitro modification of proteins, e. g. PEGylation, transglutaminase, lysyl oxidase.
• Application of biocatalysts in drug synthesis and enzyme technology, e. g. synthesis of semi-synthetic penicillins, synthesis of optically pure D-amino acids, regiospecific hydroxylation of steroids, etc.

3. Course I-PhBT.3: Advanced course on plant cell technology

• Induction and subcultivation of plant cell cultures
• Induction and cultivation of organ cultures
• Selection of cell strains by cell aggregat and protoplast cloning
• Introduction of foreign genetic material into plant cells
• Expression of foreign genetic material using plant cell and hairy root cultures
• In vitro storage of plant cell culture and meristems by cryopreservation

4. Course I-PhBT.4: Biomaterials and devices

• Biomaterial classes and their general features
• Characteristics of natural and synthetic polymers
• Biomaterial device design and preparation techniques
• Biointerfaces, surface modification
• Bioreactors
• Ex vivo and in vivo tissue engineering
• Examples of devices in Regenerative Medicine

Klausur