PD Dr. M. Brandsch

• To understand the basic principles of cell physiology. • To obtain solid knowledge on morphology and function of cell organelles. • To understand the basic techniques of in vitro animal cell cultures. • To know the problematic nature of stem cell research. • To obtain detailed knowledge on transfection of mammalian cells for biotechnological purposes. • To understand the basic principles of plant development • Knowledge of the basic concepts of in vitro plant cell, tissue and organ cultures • Insight into molecular techniques of plant cell transformation and regeneration • Overview of industrial application of plant tissue systems • Insight into biotechnological applications of transgenic plants • Survey of basic techniques of in vitro cultivation of plant cells • Skills of induction and cultivation of plant cells • Skills of handling agrobacteria in plant transformation procedures • Practical knowhow in analyzing genetically modified plants • Theoretical ability to construct a genetically modified organism by gene cloning und ex-pression in a final production host. • Advantages of bacteria, yeast and insect cells as gene expression systems. • Theoretical ability to cultivate bacteria and yeasts. • Overview over products of molecular and classic biotechnology. • Practical know-how for transformation/transfection and cultivation of plant and microbial cells

Course C.1: Animal cell biology and technology • Animal cell morphology and biochemistry • Animal cell physiology • Animal cell lines: Adherent cells, cells in suspension • Basic cell culture techniques: Trypsinization, subculturing, proliferation and viability tests • Transfection techniques • Animal cell mass culture • Special cell types: hybridoma cells
Course C.2: Plant cell technology • Plant meristems, vegetative growth and reproductive development • Basic plant regeneration and propagation procedures • Application of organ culture systems • In vitro storage and cryopreservation of plant tissues • Plant cell transformation techniques • Gene expression systems in plants • Molecular plant biotechnology: natural and novel products • Plant cell, tissue and organ cultures: initiation and maintenance • Monitoring of growth parameters and proliferation of plant cell cultures • Agrobacterium-mediated transient expression of reporter genes • Testing transgenic plants for the presence of foreign DNA by PCR • Monitoring of promoter activation based on reporter enzyme activity
Course C.3: Molecular Biotechnology • Principal mechanisms of DNA synthesis in vitro and in vivo - use of enzymes for gene cloning. • Regulation of gene expression in prokaryotes and use for heterologous expression of proteins. • Methods of gene cloning and target gene isolation. • Theoretical ability to construct a genetically modified organism for heterologous protein expression. • Theoretical ability to cultivate bacteria and yeasts. • Advantages of bacteria and yeast as gene expression systems.
Course C.4: Practical course on construction of hosts and vectors • Amplification of target DNA using PCR, restriction digest and vector ligation. • Transformation of E. coli with plasmid, selection, monitoring of the expression success.

examination on lab course contents part microorganisms, examination on lab course contents part plant cell technology, Protocol on lab course part microorganisms, Protocol on lab course part plant cell technology

Klausur