PHA.03537.03 - Purification of products of pharmaceutical biotechnology (Downstream Processing) (Complete module description)
| PHA.03537.03 | 10 CP |
|---|---|
| Module label | Purification of products of pharmaceutical biotechnology (Downstream Processing) |
| Module code | PHA.03537.03 |
| Semester of first implementation | |
| Faculty/Institute | Institut für Pharmazie |
| Module used in courses of study / semesters |
|
| Responsible person for this module | |
| Further responsible persons |
Prof. Dr. M. Pietzsch |
| Prerequisites | Module C: Construction of production organisms; Module D: Introduction to bioprocess technology |
| Skills to be acquired in this module | Knowledge of the basic concepts of downstream processing Foundations of protein purification Knowledge on equipment and design parameters Knowledge of scale-up parameters Challenges in prevention or elimination of contaminants Knowledge on process integration: Strain development, up- and downstream processing Bbasics of technical/industrial purification of proteins Know how to plan and perform the purification of a target protein from biomass |
| Module contents | Course G.1: Introduction to Downstream Processing Overview on potential contaminations of target products and strategies for their removal Equipment: Design and operation Cell disintegration Solid-Fluid separations Liquid-Liquid extraction Precipitation and crystallization Chromatographic separations Special applications: Purification of membrane proteins, inclusion bodies, antibodies, vi-ruses, DNA, etc. Course G.2: Practical course on Downstream Processing Purity control by SDS-PAGE and determination of specific enzymatic activity Cell disintegration Solid / Liquid Separation Protein precipitation Preparative chromatography for the purification of proteins Packing of chromatography columns Generation of purification tables |
| Forms of instruction |
Lecture (2 SWS)
Seminar (1 SWS) Seminar (1 SWS) Practical training (4 SWS) Course |
| Languages of instruction | German, English |
| Duration (semesters) | 1 Semester Semester |
| Module frequency | jedes Sommersemester |
| Module capacity | unlimited |
| Time of examination | |
| Credit points | 10 CP |
| Share on module final degree | Course 1: %; Course 2: %; Course 3: %; Course 4: %; Course 5: %. |
| Share of module grade on the course of study's final grade | 1 |
| Module course label | Course type | Course title | SWS | Workload of compulsory attendance | Workload of preparation / homework etc | Workload of independent learning | Workload (examination and preparation) | Sum workload |
|---|---|---|---|---|---|---|---|---|
| Course 1 | Lecture | Introduction to Downstream Processing | 2 | 0 | ||||
| Course 2 | Seminar | Introduction to Downstream Processing | 1 | 0 | ||||
| Course 3 | Seminar | Practical course on Downstream Processing | 1 | 0 | ||||
| Course 4 | Practical training | Practical course on Downstream Processing | 4 | 0 | ||||
| Course 5 | Course | Selbststudium | 0 | |||||
| Workload by module | 300 | 300 | ||||||
| Total module workload | 300 | |||||||
| Examination | Exam prerequisites | Type of examination | |
|---|---|---|---|
| Course 1 | |||
| Course 2 | |||
| Course 3 | |||
| Course 4 | |||
| Course 5 | |||
| Final exam of module | 1 attestation, 1 presentation, 1 protocol on lab course |
Klausur |
|
| Exam repetition information | |||
| Prerequisites and conditions | Prerequisites | Frequency | Compulsory attendance | Share on module grade in percent |
|---|---|---|---|---|
| Course 1 | Summer semester | No | % | |
| Course 2 | Summer semester | No | % | |
| Course 3 | Summer semester | No | % | |
| Course 4 | Summer semester | No | % | |
| Course 5 | Summer semester | No | % |
