MLU
PHA.06117.01 - H-Both: Analytical Methods (Complete module description)
Original version English
PHA.06117.01 10 CP
Module label H-Both: Analytical Methods
Module code PHA.06117.01
Semester of first implementation
Faculty/Institute Institut für Pharmazie
Module used in courses of study / semesters
  • Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation valid from WS 2019/20 > Pflichtmodule
  • Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation (WS 2015/16 - SoSe 2023) > Pflichtmodule
Responsible person for this module
Further responsible persons
Dr. M. Niepel
Prerequisites
A-Both: Introduction to Industrial and Pharmaceutical Biotechnology
Skills to be acquired in this module
  • Basics of different analytical methods including vibrational spectroscopy, separation techniques (gas and liquid chromatography, electrophoresis), NMR and mass spectrometry
  • Scope and limitations of these analytical techniques for identification and quantification
  • Applying the right tools to the right questions
  • Ability to set up a proteomics workflow in industry
  • Ability to judge the quality of results, i.e. protein identifications
  • Understanding the role of computing in knowledge discovery and apply bioinformatics tools in data processing, workflow automation, and structure determination
Module contents
Course H-Both.1: Separation Techniques, Vibrational Spectroscopy and NMR
  • Liquid Chromatography: Normal and reversed Phase (U)HPLC, Ion exchange chrom.
  • Electrophoresis: IEF, 2-DE
  • Detection Methods
  • Vibrational spectroscopy: FT-IR, NIR, Raman, and Terahertz spectroscopies
  • Multivariate analysis for qualitative (principal component analysis) and quantitative (partial least squares regression) analysis
  • NMR spectroscopy for structure elucidation and quantification
Course H-Both.2: Mass Spectrometry and Proteomics
  • Fundamentals of mass spectrometry: ionization techniques in gas, liquid and solid state, different analyzers and their performances, MS2 and MSn, elemental composition determination
  • Hyphenated techniques, quantitation via LC-MS(/MS), TripleQuad scan modes
  • Molecular profiling and imaging techniques, ion mobility spectroscopy
  • Small molecule analysis, sample preparation, qualitative and quantitative investigations via GC-FID and GC-MS, data evaluation and use of databases
  • Preprocessing of mass spectra and database searching
  • Qualitative and quantitative proteomics
  • Protein databases, annotations, and search engines
  • Characterization of PTMs
Course H-Both.3: Analytical methods
  • Methods for separating complex mixtures
  • Data-dependent acquisitions for protein mass spectrometry
  • GC-MS for the quantification of drugs
Forms of instruction Lecture (3 SWS)
Seminar (2 SWS)
Seminar (1 SWS)
Practical training (2 SWS)
Course
Languages of instruction German, English
Duration (semesters) 1 Semester Semester
Module frequency jedes Sommersemester
Module capacity unlimited
Time of examination
Credit points 10 CP
Share on module final degree Course 1: %; Course 2: %; Course 3: %; Course 4: %; Course 5: %.
Share of module grade on the course of study's final grade 1
Module course label Course type Course title SWS Workload of compulsory attendance Workload of preparation / homework etc Workload of independent learning Workload (examination and preparation) Sum workload
Course 1 Lecture Lecture 3 0
Course 2 Seminar Project seminar 2 0
Course 3 Seminar Project seminar 1 0
Course 4 Practical training Lab course 2 0
Course 5 Course Private studies 0
Workload by module 300 300
Total module workload 300
Examination Exam prerequisites Type of examination
Course 1
Course 2
Course 3
Course 4
Course 5
Final exam of module
Attestation, Protocol on project seminar H-Both.3
Klausur
Exam repetition information
Prerequisites and conditions Prerequisites Frequency Compulsory attendance Share on module grade in percent
Course 1 Summer semester No %
Course 2 Summer semester No %
Course 3 Summer semester No %
Course 4 Summer semester No %
Course 5 Summer semester No %