Modules of the first semester (A-Both, B-PhBT, C-Both, D-Both)
Kompetenzziele
Knowledge of the concepts of the immune response
Basic knowledge about organs, cells and molecules of the immune system
Knowledge, how specific cellular and humoral immune responses are developed
Basic knowledge about autoimmune diseases
Basic knowledge about allergies
Knowledge about principles of tumor immunology including knowledge about selected examples of therapeutic anti-tumor antibodies
Knowledge about principles of vaccine development: selected examples from human and veterinary medicine
Basic knowledge about production/ selection and characterization of therapeutic mono-clonal antibodies and recombinant antibodies
Overview of the different types of monoclonal antibodies (mAbs)
Knowledge of the targets for mAbs
Knowledge of the PK and PD characteristics of mAbs
Insight into mAbs under clinical development
Insight into the currently approved mAbs
Understanding of antibody production: polyclonal antibodies from mice, monoclonal antibodies from hybridoma cells, recombinant antibodies from bacteria
Understanding of antibody action: specificity, affinity, avidity
Understanding the background and basics of different immunological tests: ELISA, Western blot and hemagglutination test
Understanding the production (phages, bacteria) and physical (Western blot) and functional (ELISA) characterization of different formats of recombinant antibodies: nanobod-ies, scFv.
Diseases relevant for DNA therapy
Functional aspects of therapeutic DNA
Delivery systems for DNA
Current therapy strategies
Modulinhalte
Course K-PhBT.1 Vaccines, Immunology
Basic immunology about innate and specific immune response: Organs, cells and molecules of the immune system
Immune cell differentiation, B- and T-lymphocytes, development of the antibody and TCR repertoire
Interaction between cells and molecules in the immune system to develop specific humoral and cellular immune responses
Failures of the immune system: Basics of allergy and autoimmune disease
Basics of tumor immunology
Vaccines in application and in development
Antibodies for therapy and diagnosis
Course K-PhBT.2: Clinical development of monoclonal antibodies
Basics of immunology
Technical and methodical basics of production/selection of monoclonal antibodies by classical methods and advanced modern technologies
The selection of monoclonal recombinant antibodies of different formats by Phage Display, Screening
Production of monoclonal antibodies in CHO cells including optimization of cell lines, cultivation, media, scale up as well as purification strategies and methods.
Plant-based antibody production is generally explained with a specific focus to expression enhancement, purification tags and glycan-engineering.
Mechanisms of action of therapeutic antibodies
Antibody optimization wrt pharmacokinetical half-life extension, ADCC and CDC
Development of therapeutic anti-tumour antibodies
Approval and necessary tests to perform phase I, II and II trials.
Bleeding of immunized mice, production of sera, characterization by ELISA and Western blot
Observation of hybridoma cultures, harvest of supernantants, functional characterization by ELISA and Western blot
Production of specific display phages, nanobodies and scFv in E. coli, physical
Characterization by Western blot and functional characterization by competitive ELISA
Characterization of potentially neutralizing anti H5N1 antibodies in an hemagglutination test with plant-derived Virus-Like-Particles (VLPs)
