MLU
PHA.06110.02 - C-Both: Construction of production organisms - Hosts and vectors (Complete module description)
Original version English
PHA.06110.02 10 CP
Module label C-Both: Construction of production organisms - Hosts and vectors
Module code PHA.06110.02
Semester of first implementation
Faculty/Institute Institut für Pharmazie
Module used in courses of study / semesters
  • Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation valid from WS 2019/20 > Pflichtmodule
  • Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Version of accreditation (WS 2015/16 - SoSe 2023) > Pflichtmodule
Responsible person for this module
Further responsible persons
S. Schilling
Prerequisites
Skills to be acquired in this module
  • Knowledge on synthesis and structure/function relationship of DNA, RNA and protein
  • Basic principles of gene structure, regulatory elements, function and regulation of activity in biotechnologically important prokaryotic and eukaryotic organisms
  • Theoretical ability to cultivate bacteria, yeasts and phototrophic organisms
  • Knowledge about basic principles of gene cloning
  • Theoretical ability to construct a genetically modified organism by gene cloning und expression in a final production host
  • Insight into molecular techniques of plant and microalgae transformation
  • Survey of basic techniques of plant regeneration and concepts of in vitro plant cell, tissue and organ cultures
  • Overview of biotechnological application of phototrophic organisms and transgenic plants
  • Advantages of bacteria, yeast and insect cells as gene expression systems
  • Overview over products of molecular and classic biotechnology of microorganisms
  • Practical know-how for transfection and cultivation of microbial cells
  • Practical know-how on heterologous gene expression in microorganisms and plants
  • Practical knowhow in analyzing genetically modified plants
Module contents
Course C-Both.1: Methods and strategies for the generation of production strains
  • Overview biotechnologically relevant organisms: product related selection, advantages/disadvantages
  • Toolbox biotechnology: Gene analysis, DNA/protein sequencing, detection methods for products
  • Strategies for improvement of strains and products: random/site directed mutagenesis, protein tags
  • Examples for successful application in pharmaceutical and industrial biotechnology using different production organisms (bacteria, yeast, plants)
Course C-Both.2: Biotechnology of phototrophic organisms
  • Principal organization and expression of nuclear and organellar plant genomes
  • Plant and cell architecture, compartments and protein sorting
  • Strategies and procedures for plant regeneration and propagation
  • Plant cell, tissue and organ cultures: initiation and maintenance
  • Mechanism of Agrobacterium-mediated T-DNA transfer and applications
  • Plant transformation techniques and gene expression systems in plants
  • Vector design and optimization, stability and analysis of transgenes
  • Examples of molecular plant biotechnology: natural and novel products
  • Overview of (biotechnological relevant) micro- and macroalgae and application
  • The unicellular green algae Chlamydomonas reinhardtii as a model organism
  • Molecular biology of and gene technology with Cyanobacteria
Course C-Both.3: Molecular Biotechnology
  • Principal mechanisms of DNA synthesis in vitro and in vivo and use of enzymes for gene cloning
  • Mechanism of RNA and protein synthesis in bacteria
  • Structure and function of RNA and DNA
  • Regulation of gene expression in prokaryotes and eukaryotes (yeast) and use for heterologous expression of proteins
  • Methods of gene cloning and target gene isolation, library construction and screening
  • Theoretical ability to construct a genetically modified organism for heterologous protein production
  • Theoretical ability to cultivate bacteria and yeasts
Course C-Both.4: Project seminar on construction of hosts and vectors
  • Amplification of target DNA using PCR, restriction digest and vector ligation.
  • Transformation of E. coli with plasmid, selection, monitoring of the expression success.
  • Testing transgenic plants for the presence of transferred DNA
  • Monitoring heterologous protein production in plants
Forms of instruction Lecture (2 SWS)
Seminar (3 SWS)
Practical training (4 SWS)
Course
Languages of instruction German, English
Duration (semesters) 1 Semester Semester
Module frequency jedes Wintersemester
Module capacity unlimited
Time of examination
Credit points 10 CP
Share on module final degree Course 1: %; Course 2: %; Course 3: %; Course 4: %.
Share of module grade on the course of study's final grade 1
Module course label Course type Course title SWS Workload of compulsory attendance Workload of preparation / homework etc Workload of independent learning Workload (examination and preparation) Sum workload
Course 1 Lecture Lecture 2 0
Course 2 Seminar Project seminar 3 0
Course 3 Practical training Lab course 4 0
Course 4 Course Selbststudium 0
Workload by module 300 300
Total module workload 300
Examination Exam prerequisites Type of examination
Course 1
Course 2
Course 3
Course 4
Final exam of module
Examination on course contents part microorganisms, Protocol on course part microorganisms, Examination on course contents part plant cell technology, Protocol on course part plant cell technology
Klausur
Exam repetition information
Prerequisites and conditions Prerequisites Frequency Compulsory attendance Share on module grade in percent
Course 1 Winter semester No %
Course 2 Winter semester No %
Course 3 Winter semester No %
Course 4 Winter semester No %