PHA.06110.02 - C-Both: Construction of production organisms - Hosts and vectors (Vollständige Modulbeschreibung)

PHA.06110.02	10 CP
Modulbezeichnung	C-Both: Construction of production organisms - Hosts and vectors
Modulcode	PHA.06110.02
Semester der erstmaligen Durchführung
Fachbereich/Institut	Institut für Pharmazie
Verwendet in Studiengängen / Semestern	Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Akkreditierungsfassung gültig ab WS 2019/20 > Pflichtmodule Pharmaceutical and Industrial Biotechnology (MA120 LP) (Master) > Pharmazie PharmacIndusBiotech.MA120, Akkreditierungsfassung (WS 2015/16 - SoSe 2023) > Pflichtmodule
Modulverantwortliche/r
Weitere verantwortliche Personen	S. Schilling
Teilnahmevoraussetzungen
Kompetenzziele	Knowledge on synthesis and structure/function relationship of DNA, RNA and protein Basic principles of gene structure, regulatory elements, function and regulation of activity in biotechnologically important prokaryotic and eukaryotic organisms Theoretical ability to cultivate bacteria, yeasts and phototrophic organisms Knowledge about basic principles of gene cloning Theoretical ability to construct a genetically modified organism by gene cloning und expression in a final production host Insight into molecular techniques of plant and microalgae transformation Survey of basic techniques of plant regeneration and concepts of in vitro plant cell, tissue and organ cultures Overview of biotechnological application of phototrophic organisms and transgenic plants Advantages of bacteria, yeast and insect cells as gene expression systems Overview over products of molecular and classic biotechnology of microorganisms Practical know-how for transfection and cultivation of microbial cells Practical know-how on heterologous gene expression in microorganisms and plants Practical knowhow in analyzing genetically modified plants
Modulinhalte	Course C-Both.1: Methods and strategies for the generation of production strains Overview biotechnologically relevant organisms: product related selection, advantages/disadvantages Toolbox biotechnology: Gene analysis, DNA/protein sequencing, detection methods for products Strategies for improvement of strains and products: random/site directed mutagenesis, protein tags Examples for successful application in pharmaceutical and industrial biotechnology using different production organisms (bacteria, yeast, plants) Course C-Both.2: Biotechnology of phototrophic organisms Principal organization and expression of nuclear and organellar plant genomes Plant and cell architecture, compartments and protein sorting Strategies and procedures for plant regeneration and propagation Plant cell, tissue and organ cultures: initiation and maintenance Mechanism of Agrobacterium-mediated T-DNA transfer and applications Plant transformation techniques and gene expression systems in plants Vector design and optimization, stability and analysis of transgenes Examples of molecular plant biotechnology: natural and novel products Overview of (biotechnological relevant) micro- and macroalgae and application The unicellular green algae Chlamydomonas reinhardtii as a model organism Molecular biology of and gene technology with Cyanobacteria Course C-Both.3: Molecular Biotechnology Principal mechanisms of DNA synthesis in vitro and in vivo and use of enzymes for gene cloning Mechanism of RNA and protein synthesis in bacteria Structure and function of RNA and DNA Regulation of gene expression in prokaryotes and eukaryotes (yeast) and use for heterologous expression of proteins Methods of gene cloning and target gene isolation, library construction and screening Theoretical ability to construct a genetically modified organism for heterologous protein production Theoretical ability to cultivate bacteria and yeasts Course C-Both.4: Project seminar on construction of hosts and vectors Amplification of target DNA using PCR, restriction digest and vector ligation. Transformation of E. coli with plasmid, selection, monitoring of the expression success. Testing transgenic plants for the presence of transferred DNA Monitoring heterologous protein production in plants
Lehrveranstaltungsformen	Vorlesung (2 SWS) Seminar (3 SWS) Praktikum (4 SWS) Kursus
Unterrichtsprachen	Deutsch, Englisch
Dauer in Semestern	1 Semester Semester
Angebotsrhythmus Modul	jedes Wintersemester
Aufnahmekapazität Modul	unbegrenzt
Prüfungsebene
Credit-Points	10 CP
Modulabschlussnote	LV 1: %; LV 2: %; LV 3: %; LV 4: %.
Faktor der Modulnote für die Endnote des Studiengangs	1

Modulveranstaltung	Lehrveranstaltungsform	Veranstaltungstitel	SWS	Workload selbstgestaltete Arbeit	Workload Summe
LV 1	Vorlesung	Lecture	2		0
LV 2	Seminar	Project seminar	3		0
LV 3	Praktikum	Lab course	4		0
LV 4	Kursus	Selbststudium			0
Workload modulbezogen				300	300
Workload Modul insgesamt					300

Prüfung	Prüfungsvorleistung	Prüfungsform
LV 1
LV 2
LV 3
LV 4
Gesamtmodul	Examination on course contents part microorganisms, Protocol on course part microorganisms, Examination on course contents part plant cell technology, Protocol on course part plant cell technology	Klausur
Wiederholungsprüfung

Regularien	Angebotsrhythmus	Anwesenheitspflicht	Gewicht an Modulnote in %
LV 1	Wintersemester	Nein	%
LV 2	Wintersemester	Nein	%
LV 3	Wintersemester	Nein	%
LV 4	Wintersemester	Nein	%